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tns  (PromoCell)


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    Structured Review

    PromoCell tns
    Tns, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypsin+neutralizing+solution/Trypsin+Neutralizing+Solution/bio_rxiv__64898__2026__05__06__723334-217-17-18
    Average 94 stars, based on 83 article reviews
    tns - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma.
    Article Snippet: When confluent, RPE cells were passaged using 0.05% trypsin and neutralized by RPE medium, while NCMs were passaged using 0.025% trypsin and neutralized by Trypsin Neutralizing Solution (PromoCell).

    Subculturing Assay:

    Article Title: Glycocalyx strengthens endothelial barrier function and protects from angioedema inducing compounds
    Article Snippet: .. The splitting reagents Hepes BSS, Trypsin/EDTA, and Trypsin Neutralizing Solution (all from PromoCell GmbH, Heidelberg ) were utilized for subculturing. .. The cell culture plates and filter inserts ( Sarstedt AG & Co. KG, Nümbrecht ) used in the experiments were pre-coated with a 50 μg/ml collagen I solution ( Corning GmbH, Kaiserslautern ).

    Produced:

    Article Title: SGLT2 inhibitors attenuate cholesterol accumulation in Human Aortic Smooth Muscle Cells.
    Article Snippet: Purpose: The influence of SGLT2 inhibitors (SGLT2i) on cholesterol accumulation in Human Aortic Smooth Muscle Cells (HAoSMCs) has not yet been evaluated.. This study aimed to assess the effect of SGLT2 inhibitors on cholesterol accumulation in HAoSMCs.. Materials and methods: HAoSMCs were treated with empagliflozin (1 and 10 μM), dapagliflozin (1 and 10 μM), and canagliflozin (1 and 10 μM), with or without cholesterol–methyl-β-cyclodextrin complex (cholesterol complex), for 144 h. Lipid accumulation was assessed using Oil Red O staining, and absorbance at 492 nm was measured to quantify lipid content.

    Saline:

    Article Title: Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7
    Article Snippet: Human Umbilical Vein Endothelial Cells (HUVECs) (C-12203; PromoCell) were cultured in endothelial cell growth media supplemented with 2% (v/v) FCS (C-22110; PromoCell). .. HUVECs were washed with HEPES-buffered saline solution (C-40020; PromoCell) and detached using trypsin-EDTA (0.04%/0.03%; C-41020; PromoCell), followed by neutralization with trypsin neutralizing solution (C-41120; PromoCell). .. The human monocyte cell line THP-1 (TIB-202; ATCC; Manassas, VA, USA) was cultured in RPMI 1640 Medium (A1049101; Thermo Fisher) supplemented with 10% fetal bovine serum (FBS; S0615; Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin-streptomycin (15140122; Thermo Fisher).

    Neutralization:

    Article Title: Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7
    Article Snippet: Human Umbilical Vein Endothelial Cells (HUVECs) (C-12203; PromoCell) were cultured in endothelial cell growth media supplemented with 2% (v/v) FCS (C-22110; PromoCell). .. HUVECs were washed with HEPES-buffered saline solution (C-40020; PromoCell) and detached using trypsin-EDTA (0.04%/0.03%; C-41020; PromoCell), followed by neutralization with trypsin neutralizing solution (C-41120; PromoCell). .. The human monocyte cell line THP-1 (TIB-202; ATCC; Manassas, VA, USA) was cultured in RPMI 1640 Medium (A1049101; Thermo Fisher) supplemented with 10% fetal bovine serum (FBS; S0615; Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin-streptomycin (15140122; Thermo Fisher).



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